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Mystery solved: Tridentinosaurus antiquus, the oldest fossil reptile from the Alps is, in part, an historical forgery

Palaeontological analysis shows renowned fossil thought to show soft tissue preservation is in fact just paint

A 280-million-year-old fossil that has baffled researchers for decades has been shown to be, in part, a forgery following new examination of the remnants.

The discovery has led the team led by Dr Valentina Rossi of University College Cork, Ireland (UCC) to urge caution in how the fossil is used in future research.

Tridentinosaurus antiquus was discovered in the Italian alps in 1931 and was thought to be an important specimen for understanding early reptile evolution.

Its body outline, appearing dark against the surrounding rock, was initially interpreted as preserved soft tissues. This led to its classification as a member of the reptile group Protorosauria.

Tridentinosaurus antiquus was discovered in the Italian alps in 1931 and was thought to be an important specimen for understanding early reptile evolution - but has now been found to be, in part a forgery. Its body outline, appearing dark against the surrounding rock, was initially interpreted as preserved soft tissues but is now known to be paint. Credits: Dr Valentina Rossi
Tridentinosaurus antiquus was discovered in the Italian alps in 1931 and was thought to be an important specimen for understanding early reptile evolution – but has now been found to be, in part a forgery. Its body outline, appearing dark against the surrounding rock, was initially interpreted as preserved soft tissues but is now known to be paint. Credits: Dr Valentina Rossi

However, this new research, published in the scientific journal Palaeontology, reveals that the fossil renowned for its remarkable preservation is mostly just black paint on a carved lizard-shaped rock surface.

The purported fossilised skin had been celebrated in articles and books but never studied in detail. The somewhat strange preservation of the fossil had left many experts uncertain about what group of reptiles this strange lizard-like animal belonged to and more generally its geological history.

Dr Rossi, of UCC’s School of Biological, Earth and Environmental Sciences, said:

“Fossil soft tissues are rare, but when found in a fossil they can reveal important biological information, for instance, the external colouration, internal anatomy and physiology.”

“The answer to all our questions was right in front of us, we had to study this fossil specimen in details to reveal its secrets – even those that perhaps we did not want to know”.

Dr Valentina Rossi with an image of Tridentinosaurus antiquus. The fossil, discovered in the Italian alps in 1931, was thought to be an important specimen for understanding early reptile evolution - but has now been found to be, in part a forgery. Its body outline, appearing dark against the surrounding rock, was initially interpreted as preserved soft tissues but is now known to be paint. Credits: Zixiao Yang
Dr Valentina Rossi with an image of Tridentinosaurus antiquus. The fossil, discovered in the Italian alps in 1931, was thought to be an important specimen for understanding early reptile evolution – but has now been found to be, in part a forgery. Its body outline, appearing dark against the surrounding rock, was initially interpreted as preserved soft tissues but is now known to be paint. Credits: Zixiao Yang

The microscopic analysis showed that the texture and composition of the material did not match that of genuine fossilised soft tissues.

Preliminary investigation using UV photography revealed that the entirety of the specimen was treated with some sort of coating material. Coating fossils with varnishes and/or lacquers was the norm in the past and sometimes is still necessary to preserve a fossil specimen in museum cabinets and exhibits. The team was hoping that beneath the coating layer, the original soft tissues were still in good condition to extract meaningful palaeobiological information.

The findings indicate that the body outline of Tridentinosaurus antiquus was artificially created, likely to enhance the appearance of the fossil. This deception misled previous researchers, and now caution is being urged when using this specimen in future studies.

The team behind this research includes contributors based in Italy at the University of Padua, Museum of Nature South Tyrol, and the Museo delle Scienze in Trento.

Co-author Prof Evelyn Kustatscher, coordinator of the project “Living with the supervolcano”, funded by the Autonomous Province of Bolzano said:

“The peculiar preservation of Tridentinosaurus had puzzled experts for decades. Now, it all makes sense. What it was described as carbonized skin, is just paint”.

However all not all is lost, and the fossil is not a complete fake. The bones of the hindlimbs, in particular, the femurs seem genuine, although poorly preserved. Moreover, the new analyses have shown the presence of tiny bony scales called osteoderms – like the scales of crocodiles – on what perhaps was the back of the animal.

This study is an example of how modern analytical palaeontology and rigorous scientific methods can resolve an almost century-old palaeontological enigma.

Bibliographic information:

Forged soft tissues revealed in the oldest fossil reptile from the early Permian of the Alps, Palaeontology (16-Feb-2024), DOI: 10.1111/pala.12690

Press release from University College Cork

ARRHYTHMOGENIC CARDIOMYOPATHY: the IMPACT research project coordinated by the University of Padua has been funded with 4 million euros

The research project, named IMPACT – Cardiogenomics meets Artificial Intelligence: a step forward in arrhythmogenic cardiomyopathy diagnosis and treatment – will run for 36 months and has been funded with 4 million euros by the European Innovation Council for cardiogenomics. The mission of the European Innovation Council, established by the European Commission in 2021, is to identify and to develop innovative technologies for research.

The international team, led by Professor Alessandra Rampazzo of the Department of Biology at the University of Padua and composed of researchers from Universiteit Maastricht (Dr. Martina Calore), Universitair Medisch Centrum Utrecht (Dr. Anneline te Riele), Lutech Group (Dr. Barbara Alicino), Italbiotec Consortium (Dr. Melissa Balzarotti), Ksilink (Dr. Peter Sommer), and Italfarmaco (Dr. Christian Steinkuhler), will study the development of new therapies for arrhythmogenic cardiomyopathy (ACM), a genetic disease that affects the heart and represents one of the main causes of ventricular arrhythmias and sudden cardiac death. With an incidence of 1 in 5000, it can be considered a highly relevant cardiovascular disease.

Arrhythmogenic cardiomyopathy is a degenerative heart condition often implicated in the sudden death of athletes and adolescents. The histopathological hallmark is fibroadipose replacement of the myocardium, which impairs the functioning of the cardiac muscle, leading to the onset of ventricular arrhythmias. Currently, there is no therapy available to prevent or at least slow down the progressive tissue changes that could be ultimately fatal.

Mutations in numerous disease genes have been identified as contributors to this condition. However, many of the genetic alterations found in the DNA of affected patients have uncertain significance and are not yet directly linked to the disease, providing limited utility for both geneticists and physicians.

“Thanks to the funding obtained from Horizon Europe, our research project aims to open up new therapeutic perspectives based on the results obtained from various disease models. This is an innovative and multidisciplinary project, whose success is strongly supported by the diverse but complementary expertise of partners from academic institutions and leading companies in the fields of computer science, biotechnology, and pharmaceuticals,” says Professor Alessandra Rampazzo of the Department of Biology at the University of Padua, scientific coordinator of the international team. “Such collaboration will allow us to achieve our ambitious goals. The overall objective of the project funded by the European Community is to integrate and analyze clinical and molecular data from the ACM patient registry using artificial intelligence, along with data from structural and functional analyses of cellular models such as three-dimensional cardiac microtissues and in vivo models. These results will enable us to gain a better understanding of the role and impact of genetic alterations on the clinical progression of arrhythmogenic cardiomyopathy. Additionally,” Alessandra Rampazzo concludes, “the project includes screening and subsequent evaluation of the therapeutic potential of several compounds and innovative molecules, both in cellular and animal models.”

Alessandra Rampazzo arrhythmogenic cardiomyopathy
Alessandra Rampazzo, leading the IMPACT research project

The discovery of new therapeutic targets and an understanding of the underlying pathogenic mechanisms could not only lead to new therapies for ACM but could also pave the way for better clinical management of the disease and an improvement in the quality of life for patients.

The meeting of all participants, which will officially launch the project, will be held in Padua on October 26th and 27th.

 

Press release from the University of Padua

COMPUTER MODELS OF INJURED BRAINS PREDICT NEUROLOGICAL DEFICITS IN STROKE

The fundamental mechanisms underlying the dynamics of brain activity are still largely unknown. Their knowledge could help understand the brain‘s response to pathological conditions, such as brain injury (strokes). Despite the efforts of the scientific community, the neural mechanisms underlying the functional and behavioral recovery of stroke patients are still poorly understood.

The study Recovery of neural dynamics criticality in personalized whole brain models of stroke published in Nature Communications, fruit of an international collaboration between physicists, neurologists, and psychologists, by Rodrigo Rocha, Loren Koçillari, Samir Suweis, Michele De Grazia, Michel Thiebaut De Schotten, Marco Zorzi and Maurizio Corbetta, proposes the theory of brain criticality to explain brain-behaviour relationships in neurological patients.

Interdisciplinary research in neuroscience, inspired by statistical physics, has suggested that healthy brain’s neural dynamics operate at a critical state (i.e., in the vicinity of a critical phase transition between order and disorder) that provides optimal functional capabilities. If criticality is indeed a fundamental property of healthy brains, then neurological dysfunctions shall alter this optimal dynamical configuration. Some studies have reported disrupted criticality during epileptic seizures, slow-wave sleep, anesthesia, and Alzheimer’s disease. However, a crucial test of the hypothesis requires showing alterations of criticality after focal brain injury that cause local alterations of the brain’s structural and functional architecture. Furthermore, these alterations shall improve over time in parallel to recovery. Another prediction is that if criticality is essential for behaviour, then its alteration after focal injury shall relate to behavioural dysfunction and recovery of function. Finally, changes in criticality should also correlate with plasticity mechanisms that underlie recovery.

“The aim of the present work was to address these important questions through an interdisciplinary approach combining neuroimaging, computational neuroscience, statistical physics, and data science methods“, explain Rodrigo Rocha (Department of Physics of the Federal University of Santa Catarina, Florianópolis, Brazil). “We examined how brain lesions change criticality using a novel personalized whole brain modelling approach. Our theoretical framework models individual (i.e., single patient) brain dynamics based on real structural connectivity networks. We studied longitudinally a cohort of first-time stroke and healthy participants with neuropsychological tests, and diffusion weighted imaging (DWI) and functional MRI (fMRI) connectivity measures. We found that patients affected by stroke present at three months decreased levels of neural activity, decreased entropy, and decreased strength of functional connections. All these factors contribute to an overall loss of criticality that improves over time with recovery. We also show that changes in criticality predict the degree of behavioural recovery and critically depend on specific white matter connections. In summary, our work describes an important advance in understanding the alteration of brain dynamics as well as brain-behaviour relationships in neurological patients” says Rodrigo Rocha.

Rodrigo Rocha

“Our results show that personalized whole brain computer models can be used to track and predict stroke recovery at the level of single patients, thereby opening promising paths for novel interventions as computer models will allow to test the efficacy of different strategies to improve function” concludes Maurizio Corbetta (Department of Neuroscience and Padova Neuroscience, University of Padua, and Venetian Institute of Molecular Medicine).

Maurizio Corbetta fisica neuroscienze
Maurizio Corbetta

R.P.R. was funded by Research, Innovation and Dissemination Center for Neuromathematics (FAPESP) and the National Council for Scientific and Technological Development (CNPq), Brasil, to RR; M.T.dS by European Research Council (ERC) H2020 (grant# 818521); M.D.F. and M.Z. the Italian Ministry of Health (Grant# RF-2013-02359306); M.C. by the Italian Ministry of Research Departments of Excellence (2017-2022), CARIPARO foundation (Grant #55403), Italian Ministry of Health (Grant# RF-2018-12366899; RF-2019-12369300), H2020-SC5-2019-2 (Grant # 869505);  H2020-SC5-2019-2 (Grant # 869505).

Info sheet

Who: Department of Physics of the Federal University of Santa Catarina; Laboratory of Neural Computation Istituto Italiano di Tecnologia (Rovereto); Department of Physics and Astronomy of the University of Padua; Brain Connectivity and Behaviour Laboratory of the Sorbonne Universities; Groupe d’imagerie Neurofonctionnelle of the University of Bordeaux; Department of General Psychology of the University of Padua; IRCCS San Camillo Hospital Venice; Department of Neuroscience and Padova Neuroscience Center (PNC), of the University of Padua; and, Veneto Institute of Molecular Medicine (VIMM)

What: Interdisciplinary approach to study the role of brain criticality and brain-behavior relationships in neurological patients published in Nature Communications: Recovery of neural dynamics criticality in personalized whole brain models of stroke.

Teorie della Fisica applicate alle Neuroscienze computer models brains stroke
Computer models of injured brains predict neurological deficits in stroke. Picture by Gerd Altmann

Press release from the University of Padua.

Cognitive Aging: Work helps our brain

A new paper by SISSA and University of Padua on a large sample of the Italian population shows that occupation influences the course of cognitive decline

Invecchiamento cognitivo lavoro Cognitive Aging Work
Photo credits Claudio Schwarz on Unsplash

Trieste, 9 Dec 2021 – A recent study shows that work plays an active role in keeping our brains healthy. “We have demonstrated the role of working activity on cognitive performance”. Professor Raffaella Rumiati says. She is cognitive neuroscientist at SISSA and author of the paper Protective factor for Subjective Cognitive Decline Individuals: Trajectories and change in a longitudinal study with Italian seniors, recently published in the European Journal of Neurology. DOI: https://doi.org/10.1111/ene.15183

“Many studies have been focused on the factors influencing our brain aging and differences in cognitive decline have been often observed in association with education or other related to quality of life. From our analysis it emerges that the type of work activity also contributes to the differences in normal and pathological cognitive aging”.

The analysis: resistant and declining brains

The research, carried out by a team of scientists from the University of Padua (Dip. FISPPA), SISSA – Scuola Internazionale di Studi Superiori Avanzati and IRCSS San Camillo Hospital in Venice, quantified the relative contribution of demographic factors (age and sex), comorbidity, education and occupation to the so-called cognitive reserve, that is brain’s resistance to a damage caused by illness or aging. Participants were assessed with a series of neuropsychological tests and subsequently divided into three types of profiles based on the results: subjects at risk of cognitive decline, subjects with mild decline and subjects with severe decline.

The tests were repeated twice a few years apart. Depending on whether they maintained or worsened their profile based on their initial performance, participants were classified as “resistant” or “declining”.

Education and occupation to stay young

The analysis surprisingly shows that occupation is a good predictor of participants’ performance in addition to age and education, two factors that have been already studied.

Professor Sara Mondini of the University of Padua says:

“We confirmed that education protect people from the risk of cognitive decline and that these individuals had held more complex occupations than the individuals of the other two groups, the subjects with mild and advanced cognitive decline. Furthermore, the study showed how “resistant” group has on average higher levels of education and more complex jobs than the “declining” group.”

The results demonstrate benefits of cognitive mobilization promoted by lifelong learning and that social connection, ongoing sense of purpose and ability to function independently largely affect cognitive health and general well-being along the trajectories of aging.

Press release from the University of Padua and SISSA – Scuola Internazionale Superiore di Studi Avanzati.